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Thread: CBD cream ameliorates EAE in mice

  1. #1
    Distinguished Community Member SuzE-Q's Avatar
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    Default CBD cream ameliorates EAE in mice

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618347/

    A new formulation of cannabidiol in cream shows therapeutic effects in a mouse model of experimental autoimmune encephalomyelitis

    Sabrina Giacoppo, Maria Galuppo, [...], and Emanuela Mazzon

    Abstract
    Background
    The present study was designed to investigate the efficacy of a new formulation of alone, purified cannabidiol (CBD) (>98 %), the main non-psychotropic cannabinoid of Cannabis sativa, as a topical treatment in an experimental model of autoimmune encephalomyelitis (EAE), the most commonly used model for multiple sclerosis (MS). Particularly, we evaluated whether administration of a topical 1 % CBD-cream, given at the time of symptomatic disease onset, could affect the EAE progression and if this treatment could also recover paralysis of hind limbs, qualifying topical-CBD for the symptomatic treatment of MS.

    Methods
    In order to have a preparation of 1 % of CBD-cream, pure CBD have been solubilized in propylene glycoland basic dense cream O/A. EAE was induced by immunization with myelin oligodendroglial glycoprotein peptide (MOG35–55) in C57BL/6 mice. After EAE onset, mice were allocated into several experimental groups (Naïve, EAE, EAE-1 % CBD-cream, EAE-vehicle cream, CTRL-1 % CBD-cream, CTRL-vehicle cream). Mice were observed daily for signs of EAE and weight loss. At the sacrifice of the animals, which occurred at the 28th day from EAE-induction, spinal cord and spleen tissues were collected in order to perform histological evaluation, immunohistochemistry and western blotting analysis.

    Results
    Achieved results surprisingly show that daily treatment with topical 1 % CBD-cream may exert neuroprotective effects against EAE, diminishing clinical disease score (mean of 5.0 in EAE mice vs 1.5 in EAE + CBD-cream), by recovering of paralysis of hind limbs and by ameliorating histological score typical of disease (lymphocytic infiltration and demyelination) in spinal cord tissues. Also, 1 % CBD-cream is able to counteract the EAE-induced damage reducing release of CD4 and CD8α T cells (spleen tissue localization was quantified about 10,69 % and 35,96 % of positive staining respectively in EAE mice) and expression of the main pro-inflammatory cytokines as well as several other direct or indirect markers of inflammation (p-selectin, IL-10, GFAP, Foxp3, TGF-β, IFN-γ), oxidative injury (Nitrotyrosine, iNOS, PARP) and apoptosis (Cleaved caspase 3).

    Conclusion
    All these data suggest an interesting new profile of CBD that could lead to its introduction in the clinical management of MS and its associated symptoms at least in association with current conventional therapy.

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  3. #2
    Distinguished Community Member SalpalSally's Avatar
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    Dang Mices!!!

    Thanks SuZe!!
    Last edited by SalpalSally; 10-28-2015 at 09:44 AM.
    Love, Sally


    "The best way out is always through". Robert Frost







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  5. #3
    Distinguished Community Member Lazarus's Avatar
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    Thanks. I appreciate all posts people make about research.
    Linda
    Linda~~~~

    Be the kind of woman that when your feet hit the floor each morning the devil says:"Oh Crap, She's up!"

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    Distinguished Community Member agate's Avatar
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    Another bunch of wondrously recovering mice. Mice have all the luck. Getting cured of their MS-like disease and without even having to worry about insurance coverage.
    MS diagnosed 1980. Avonex 2002-2005. Copaxone 6/07 - 5/10.
    Member of this MS board since 2001.

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  9. #5
    Distinguished Community Member Howie's Avatar
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    Squeak, squeak!
    Roswell was a gift.

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  11. #6
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    Too funny Agate and Howie! I still don't want to be one.
    Virginia

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  13. #7
    Distinguished Community Member SuzE-Q's Avatar
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    Grassman would be so proud of this research.

    I'd be tempted to try to find a source and experiment with it myself. Shouldn't be that expensive.

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  15. #8
    Distinguished Community Member nuthatch's Avatar
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    I'd volunteer to be a research subject myself but that "sacrifice" clause is a deal breaker!
    Poor little mousies, just when things were starting to look up for them!

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  17. #9
    Distinguished Community Member agate's Avatar
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    Oops, I missed the part about "the sacrifice of the animals." Sounds grim for the mice after all.

    "Sacrifice" sounds like a possible translation problem. I think the article was translated from Italian. Maybe "killing" sounded too, um, realistic?

    However, they were trying to be kind to the mice:

    Ethics statement

    This study was carried out in strict accordance with the recommendations in the guide for the care and use of laboratory animals of the National Institutes of Health. The protocol was approved by the Ministry of Health “General Direction of animal health and veterinary drug” (Authorization 150/2014-B 28/03/2014). In particular, animal care was in compliance with Italian regulations on protection of animals used for experimental and other scientific purposes (D.M. 116/92) as well as with the EEC regulations (O.J. of E.C.L 358/1 12/18/1986). Also, it was minimized number of animals used for this experiment and their suffering.
    And:

    At the end of the experiment, which occurred at the 28th day from EAE-induction, animals were euthanized with ip of Tanax (5 ml/kg body weight).
    MS diagnosed 1980. Avonex 2002-2005. Copaxone 6/07 - 5/10.
    Member of this MS board since 2001.

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  19. #10
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    Maybe they should find Pizza Rat in New York and try it on him. He seems pretty strong!
    Virginia

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